Things to be wary of when using PEDEL:

PEDEL uses a generic Poisson model of sequence mutations. There are a couple of simplifications that you should be aware of: Please refer to Patrick W. M., Firth A. E., Blackburn J.M., 2003, User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries, Protein Eng., 16, 451-457 for further discussion of PEDEL.

A good review of the sources of bias in epPCR (and other directed evolution protocols) can be found in Neylon C., 2004, Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution, Nucleic Acids Res., 32, 1448-1459.

Return to PEDEL server page.
Return to PEDEL software download page.