Things to be wary of when using DRIVeR:

DRIVeR uses a generic Poisson model of crossover probabilities and positions. There are a few caveats that you should be aware of: Please refer to Patrick W. M., Firth A. E., Blackburn J.M., 2003, User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries, Protein Eng., 16, 451-457 for further discussion of DRIVeR.

A good review of the sources of bias in recombination and other directed evolution protocols can be found in Neylon C., 2004, Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution, Nucleic Acids Res., 32, 1448-1459.

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Return to DRIVeR software download page.