GLUE-IT: amino acid version of GLUE for up to 6 variable codons

Note:This page is a work in progress and does not currently do anything.

Version 2008-01-20/06:06:44.

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Programme to find the expected amino acid completeness of a given library (not counting any variants with introduced stop codons) where the sequences in the library are chosen at random from a set of XYZ codon variants (where X,Y,Z are standard nucleotide codes chosen by the user, e.g. XYZ = NNS [N = A/C/G/T; S = C/G; 32 possible equiprobable codon variants; 20 + 1 possible non-equiprobable amino acid/stop codon variants]). Up to six codons may be independently varied.

To calculate the library size required in order to obtain a given completeness, or to have a given probability of being 100% complete, just try entering different library sizes and check the resulting library statistics until you home in on your required values.

Go to form for single codon variants.



(Leave '-'s as appropriate if varying < 6 codons.)

Codon 1:   

Codon 2:   

Codon 3:   

Codon 4:   

Codon 5:   

Codon 6: