PEDEL-AA: Programme for Estimating Amino Acid Diversity in Error-prone PCR Libraries

Version 2008-01-25/07:59:58.

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Notes on the PEDEL-AA algorithms.


Input:

 

Paste in your nucleotide sequence:

(Replace with your own sequence; raw text without any header line; just the part you want to mutate; make sure it is in the amino acid frame and has no in-frame stop codons!)

Maximum sequence length is 50000 nt.

Nucleotide mutation matrix (non-negative numbers):

(Overall scaling is unimportant as this is taken from the 'mean number of substitutions per daughter sequence' parameter.)

FROM
TO
  T C A G
T  
C  
A  
G  

These are (see note).

.

Use or (see note; PCR efficiency calculator).

If 'PCR distribution' selected: , .

Indel parameters:

 


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